Carbon-felt-based Bioelectrocatalytic Flow-detectors: Role of Ultrasound Irradiation during the Adsorption of Horseradish Peroxidase and Thionine for a Highly Sensitive Amperometric Determination of H<sub>2</sub>O<sub>2</sub>
نویسندگان
چکیده
منابع مشابه
Carbon-felt-based bioelectrocatalytic flow-detectors: role of ultrasound irradiation during the adsorption of horseradish peroxidase and thionine for a highly sensitive amperometric determination of H2O2.
This study reports on the first example, to our knowledge, of the usefulness of an ultrasound (US)-irradiation during an enzyme adsorption step, for enhancing the performance of a redox-enzyme-based amperometric biosensor. Horseradish peroxidase (HRP) and thionine (Th) were coadsorbed from a mixed aqueous solution of HRP and Th onto a carbon-felt (CF) under US-irradiation for 5 min with an ultr...
متن کاملCarbon-felt-based bioelectrocatalytic flow-detectors: optimization of the adsorption conditions of horseradish peroxidase and thionine onto carbon-felt for highly sensitive amperometric determination of H2O2.
Horseradish peroxidase (HRP) and thionine (Th) were co-adsorbed onto a porous carbon-felt (CF) from a mixed solution of HRP and Th to fabricate a CF-based bioelectrocatalytic flow-detector for H(2)O(2). The resulting HRP and Th co-adsorbed CF (HRP/Th-CF) was successfully used as a working electrode unit of a highly sensitive amperometric flow-H(2)O(2)-biosensor. Differing from the ordinary medi...
متن کاملCarbon felt-based bioelectrocatalytic flow detectors: highly sensitive amperometric determination of hydrogen peroxide using adsorbed peroxidase and thionine.
Horseradish peroxidase (HRP) and thionine (TN) were co-adsorbed onto a porous carbon felt (CF), and the resulting HRP and TN-adsorbed CF (HRP-TN-CF) was successfully used as a working electrode unit of a novel bioelectrocatalytic flow detector for a highly sensitive amperometric determination of hydrogen peroxide (H(2)O(2)). Co-adsorbed TN was essential to enhance the cathodic peak current of H...
متن کاملCarbon Felt-Based Bioelectrocatalytic Flow-Through Detectors: 2,6-Dichlorophenol Indophenol and Peroxidase Coadsorbed Carbon-Felt for Flow-Amperometric Determination of Hydrogen Peroxide
2,6-dichlorophenol indophenol (DCIP) and horseradish peroxidase (HRP) were coadsorbed on a porous carbon felt (CF) from their mixed aqueous solution under ultrasound irradiation for 5 min. The resulting DCIP and HRP-coadsorbed CF (DCIP/HRP-CF) showed an excellent bioelectrocatalytic activity for the reduction of H₂O₂. The coadsorption of DCIP together with HRP was essential to obtain larger bio...
متن کاملthe innovation of a statistical model to estimate dependable rainfall (dr) and develop it for determination and classification of drought and wet years of iran
آب حاصل از بارش منبع تأمین نیازهای بی شمار جانداران به ویژه انسان است و هرگونه کاهش در کم و کیف آن مستقیماً حیات موجودات زنده را تحت تأثیر منفی قرار می دهد. نوسان سال به سال بارش از ویژگی های اساسی و بسیار مهم بارش های سالانه ایران محسوب می شود که آثار زیان بار آن در تمام عرصه های اقتصادی، اجتماعی و حتی سیاسی- امنیتی به نحوی منعکس می شود. چون میزان آب ناشی از بارش یکی از مولفه های اصلی برنامه ...
15 صفحه اولذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Sciences
سال: 2011
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.27.605